TUTORIAL EXERCISE

on Diffusion, gradients and potentials

Answers to questions.

In all cases there is diffusion of ions in both directions if the permeability of the membrane allows. But where there is a concentration difference, there will be more net movement from the area of high concentration.

1.

Diffusion of K+ and Cl- ions results in a net movement from A to B, until equilibrium is reached at 55mmol on each side.

Back to Q.1




2.

In net terms, K+ moves down the concentration gradient (from A to B).

So, in terms of electrical charge, A becomes less positive and B more positive because the Cl- cannot diffuse.

This gives an electrical gradient, with the relative negativity of A attracting K+ ions to A from B.

Eventually, equilibrium reached as balance of concentration and potential gradients.

Back to Q.2



3.

No net movement of Cl-, as same concentration in A and B.

K+ will diffuse more from B to A, down its concentration gradient - so A becomes more positive and B relatively negatively charged.

This gives an electrical gradient attracting K+ to B from A.

Eventually, equilibrium reached as balance of concentration and potential gradients for K+.

Area A is electrically positive to area B.

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4.

Same as in 3, but there will also be a slower net movement of Na+, so that eventually the electrical and concentration gradients will disappear as both Na+ and K+ reach the same concentration (50mmol) in areas A and B.

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5.

A Na+ pump is needed to counteract the slow "leak" of Na+ ions.

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6.

Just the opposite of 3.

Na+ ions diffusing from A to B and eventual balance between electrical and concentration gradients, with area B electrically positive relative to area A.

Back to Q.6

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